Procell

Procell Cytogenetic Reagents

Pre-Hypotonic Solution
Product Code: GGS-JL007

Is to be used before the hypotonic stage of a blood or bone marrow harvest. Pre-hypotonic solution makes the hypotonic step more effective by circumventing the tendency of chromosomes to clump or to under-spread. The clumping of metaphases during the harvesting procedure often results in the loss of useful metaphases or the loss of entire cultures. Procedures such as FISH that require good well spread metaphases are often hampered by this problem.

Pre-Hypotonic Solution was developed to address this clumping problem. Optimal metaphase swelling can therefore subsequently be achieved by your hypotonic solution. Pre-Hypotonic Solution has been found to be particularly useful for template CGH metaphases. CGH hybridisations have been improved by Pre-Hypotonic Solution.

Pack size – 5 ml vial containing stock solution

PRODUCT DESCRIPTION

Pre-Hypotonic Solution is to be used before the hypotonic stage of a blood or bone marrow harvest. Pre-hypotonic Solution makes the hypotonic step more effective by circumventing the tendency of chromosomes to clump or to under-spread. The clumping of metaphases during the harvesting procedure often results in the loss of useful metaphases or the loss of entire cultures. Procedures such as FISH that require good well spread metaphases are often hampered by this problem. Pre-Hypotonic Solution was developed to address this clumping problem. Optimal metaphase swelling can therefore subsequently be achieved by your hypotonic solution.

STORAGE
Store product at 4°C for not more than 12 months
Never heat or freeze.
For laboratory research use only.

Pre Hypotonic Solution Preparation

  1. Add the contents of the supplied 5 ml vial to a 1000 ml volumetric flask
  2. Bring to 1000ml with milli-Q water or reverse osmosis-filtered deionised water

PROCEDURE 1

  1. After colchicine/colcemid/Metaphase Arresting Solution incubation is complete, spin down the culture discarding the supernatant.
  2. Mix the cell pellet well and add 10 ml of culture media, followed by 1 ml of Pre Hypotonic Solution. Mix well.
  3. Centrifuge for 10 minutes at 1000 rpm
  4. Discard the supernatant; proceed with the harvest as normal from, and including, the hypotonic step.

PROCEDURE 2

  1. Pipette out the old medium.
  2. Add 10mls of fresh medium, followed by 1ml of prehypotonic.
  3. Wait for 10 minutes.
  4. Pipette out the prehypotonic.
  5. Harvest as usual.

QUALITY CONTROL
All batches are tested on established lymphoid/myeloid cell-lines with spreading assessed using phase contrast microscopy.

MATERIAL SAFETY DATA SHEET
Date of issue 5th May 2005

IDENTIFICATION OF THE SUBSTANCE AND OF THE COMPANY

Catalogue Number: GGS-JL-007 - Pre-Hypotonic Solution

For laboratory and research purposes only

Genial Genetic Solutions Limited
Champions Business Park, Arrowe Brook Road, Upton, Wirral, UK. CH49 0AB United Kingdom
Tel: +44 (0)151 641 8810
Fax: +44 (0)151 641 8811
Email: info@genialgenetics.com
Web: www.genialgenetics.com

COMPOSITION AND COMPONENT DATA
Proprietary product containing phosphate balanced saline and enzymes/coenzyme mixture.

HAZARDS IDENTIFICATION
Non-hazardous

FIRST AID MEASURES
In case of eye contact flush eyes with copious amounts of water.
In case of skin contact immediately wash skin with soap and water.
If swallowed, wash out mouth with water and drink lots of fluids.

FIRE FIGHTING MEASURES
Not flammable.

ACCIDENTAL RELEASE MEASURES
Wear a lab coat and rubber gloves, wipe with paper tissues and place in plastic bag for proper disposal.

HANDLING AND STORAGE
Keep container tightly closed when not in use.
Store in refrigerator away from any light at 4°C
Do not freeze.

EXPOSURE CONTROL AND PERSONAL PROTECTION
Only work in a Class 2 Biological Safety Cabinet.
Always wear a lab coat and rubber gloves.
Wash hands after handling.

PHYSICAL AND CHEMICAL PROPERTIES
Appearance: Clear Liquid
Boiling Point: As for water
Vapour Pressure (pascals): As for water
Specific Gravity: 1.2
Flashpoint: N/A
Flammability Limits (%): N/A

STABILITY AND REACTIVITY

Stability: Stable
Conditions to avoid: Not available
Incompatibility: Not available
Hazardous Decomposition By-products: Nil
Hazardous Polymerisation: Not available

TOXICOLOGICAL INFORMATION
To the best of our knowledge, the chemical, physical and toxicological properties have not been thoroughly investigated.
Not a known carcinogen.

ECOLOGICAL INFORMATION
Data not yet available

ECOLOGICAL INFORMATION
Data not yet available

DISPOSAL CONSIDERATIONS
Use approved government contractor.
Observe all International, National, State and Local environmental laws.

TRANSPORT INFORMATION
Non-hazardous for transport

REGULATION
Not Applicable

OTHER INFORMATION
The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. Genial Genetic Solutions Ltd shall not be held liable for any damage resulting from handling or from contact with the above product.