Procell Cytogenetic Reagents

Optimal Hypotonic Solution
Product Code: GGS-JL005a (250ml)

Optimal Hypotonic Solution is a newly formulated hypotonic solution which achieves optimum metaphase swelling (spreading) from difficult cells that are prone to be either under-spread or overspread, and where conventional hypotonics achieve inadequate cell swelling. Optimal Hypotonic Solution increases cell volume, allowing the chromosomes more room to spread out. Well spread chromosomes with few cross-overs greatly facilitate analysis. Although developed for acute leukaemia bone marrows and tumour specimens, it has also proved useful with problem blood harvests.

Pack size – to make 250 mls

PRODUCT DESCRIPTION

Optimal Hypotonic Solution is a newly formulated hypotonic solution which achieves optimum metaphase swelling (spreading) from difficult cells that are prone to either under-spread or overspread, and where conventional hypotonics achieve inadequate cell swelling. Optimal Hypotonic Solution increases cell volume allowing the chromosomes more room to spread out. Well spread chromosomes with few cross-overs greatly aid analysis. Although developed for acute leukaemia bone marrows and tumour specimens, it has also proved useful with problem blood harvests.

STORAGE
Store the dissolved product at 4°C away from UV light for no more than 2 weeks.
The powdered product can be stored at Room Temperature for 12 months.
Never heat or freeze.
For laboratory research use only.

PROCEDURE

1. Add the contents of the supplied bottle to a 250ml volumetric flask.
2. Bring to 250ml with milli-Q water or reverse osmosis-filtered deionised water.
3. Store at 4°C in a clean glass bottle, noting down the date on the bottle.
4. During harvest replace your existing hypotonic solution with Optimal Hypotonic Solution.
5. Replace your existing hypotonic and incubate cells at 37 degrees C for a maximum of 30 minutes.
6. Add approximately 0.5ml (several pipette drops) of fresh fixative (normally 3:1 methanol:acetic acid ratio or whatever is the laboratory standard ratio) before centrifuging.
7. Continue with your usual harvesting protocol.

QUALITY CONTROL
All batches are tested on established lymphoid/myeloid cell-lines with spreading assessed using phase contrast microscopy.

MATERIAL SAFETY DATA SHEET
Date of issue 5th May 2005

IDENTIFICATION OF THE SUBSTANCE AND OF THE COMPANY

Catalogue Number: GGS-JL-005/a - Optimal Hypotonic Solution

Genial Genetic Solutions Ltd
Champions Business Park, Arrowe Brook Road, Upton, Wirral, CH49 0AB United Kingdom
Tel: +44 (0)1244 404 970
Fax: +44 (0)1244 404 971
Email: info@genialgenetics.com
Web: www.genialgenetics.com

COMPOSITION AND COMPONENT DATA
Proprietary product, an aqueous solution containing a complex mixture of organic ingredients, with sodium and other salts.

HAZARDS IDENTIFICATION
Not harmful by inhalation, with skin contact or if swallowed.

FIRST AID MEASURES
In case of eye contact flush eyes with copious amounts of water.
In case of skin contact immediately wash skin with soap and water.
If swallowed, wash out mouth with water and drink lots of fluids.

FIRE FIGHTING MEASURES
Not applicable

ACCIDENTAL RELEASE MEASURES
Wear a lab coat and rubber gloves, wipe with paper tissues and place in plastic bag for proper disposal.

HANDLING AND STORAGE
Keep container tightly closed when not in use.
Store prepared working solution at 4°C

EXPOSURE CONTROL AND PERSONAL PROTECTION
Only work in a Class 2 Biological Safety Cabinet.
Always wear a lab coat and rubber gloves.
Wash hands after handling.

PHYSICAL AND CHEMICAL PROPERTIES
Appearance: white powder
Boiling Point: As for water (prepared working solution)
Vapour Pressure (pascals): As for water (prepared working solution)
Specific Gravity: 1.4 (prepared working solution)
Flashpoint: N/A
Flammability Limits (%): N/A
Incompatible with strong oxidising agents
Incompatible with acids
Do not freeze

STABILITY AND REACTIVITY
Not applicable

TOXICOLOGICAL INFORMATION
To the best of our knowledge, the chemical, physical and toxicological properties have not been thoroughly investigated.
Not a known carcinogen.

ECOLOGICAL INFORMATION
Data not yet available

DISPOSAL CONSIDERATIONS
Use approved government contractor.
Observe all International, National, State and Local environmental laws.

TRANSPORT INFORMATION
Not applicable

REGULATORY INFORMATION
Not applicable

OTHER INFORMATION
The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. Genial Genetic Solutions Ltd shall not be held liable for any damage resulting from handling or from contact with the above product.