Procell

Procell Cytogenetic Reagents

Erythrocyte Lysis Solution (ELS)
Product Code: GGS-JL-009

Erythrocyte Lysis Solution is a simple pretreatment of heavily blood-stained amniotic fluid samples which quickly removes heavy red blood cell (erythrocyte) contamination. Unlike may similar products used in molecular biology that lysis red blood cells, this product is extremely gentle in its action and is specially suited for cell culture work. It is well documented that high concentrations of red blood cells inhibit growth of cells from amniotic fluid by restricting colony size. This product will not prevent maternal contamination from occurring because it has no effect on white blood cells.

Pack size – Concentrated stock solution to make up 1 litre

PRODUCT DESCRIPTION

Erythrocyte Lysis Solution is a simple pretreatment of heavily blood-stained amniotic fluid samples which quickly removes heavy red blood cell (erythrocyte) contamination. Unlike many similar products used in molecular biology that lyse red blood cells, this product is extremely gentle in its action and is specially suited for cell culture work. It is well documented that high concentrations of red blood cells inhibit growth of cells from amniotic fluid by restricting colony size. This product will not prevent maternal contamination from occurring because it has no effect on white blood cells.

PROCEDURE

  1. To make the working solution aseptically dissolve the supplied stock solution in sterile water up to 1 litre. Mix well before use.
  2. NOTE: Erythrocyte Lysis Solution is a supersaturated solution and there may be solid particles visible in the concentrated stock solution. These will dissolve once the solution is made up.
  3. Centrifuge 10ml of amniotic fluid for 10 minutes at 900-1000rpm.
  4. Remove supernatant aseptically and resuspend in 2 to 3ml of
  5. Erythrocyte Lysis Solution and mix gently.
  6. Centrifuge at 1500 rpm for 10 minutes.
  7. Remove supernatant and wash in 3 mls of culture media to remove any residual Erythrocyte Lysis Solution.
  8. Centrifuge again then resuspend in your medium of choice and set up in culture

QUALITY CONTROL
All batches are tested on red blood cells for lysis activity, with morphology assessed under phase microscopy.

MATERIAL SAFETY DATA SHEET
Date of issue 5th May 2005

IDENTIFICATION OF THE SUBSTANCE AND OF THE COMPANY

Catalogue Number: GGS-JL-009 - Metaphase Arresting Solution

For laboratory and research purposes only, lysing erythrocytes

Genial Genetic Solutions Ltd
Champions Business Park, Arrowe Brook Road, Upton, Wirral, CH49 0AB United Kingdom
Tel: +44 (0)1244 404 970
Fax: +44 (0)1244 404 971
Email: info@genialgenetics.com
Web: www.genialgenetics.com

COMPOSITION AND COMPONENT DATA
Prepared or working solution, sterile 0.85% ammonium chloride in phosphate buffered saline

HAZARDS IDENTIFICATION
Non-hazardous

FIRST AID MEASURES
In case of eye contact, flush eyes with copious water
In case of skin contact wash immediately with soap and water
If swallowed, rinse mouth with water and drink large amounts of fluid

FIRE FIGHTING MEASURES
Not applicable

ACCIDENTAL RELEASE MEASURES
Wearing rubber gloves, mop up with paper tissues and place in plastic bag for proper disposal

HANDLING AND STORAGE
Keep unused containers tightly closed
Store product at room temperature not more than 12 months, in the dark
For Laboratory research use only.

EXPOSURE CONTROL AND PERSONAL PROTECTION
Only work in a Class2 biological safety cabinet.
Always wear a lab coat and rubber gloves.
Wash hands after handling

PHYSICAL AND CHEMICAL PROPERTIES
Dilute aqueous solution
Appearance: clear to slightly cloudy liquid.
Boiling Point: As for water
Vapour Pressure (pascals): As for water
Specific Gravity: 1.1
Flashpoint: N/A
Flammability Limits (%): N/A

STABILITY AND REACTIVITY
Not applicable

TOXICOLOGICAL INFORMATION
Not applicable (Non-toxic)

ECOLOGICAL INFORMATION
Rapidly biodegraded

DISPOSAL CONSIDERATIONS
Use approved government contractor.
Observe all International, National, State and Local environmental laws.

TRANSPORT INFORMATION
Non-hazardous for transport

REGULATION
Not applicable

OTHER INFORMATION
The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. Genial Genetic Solutions Ltd shall not be held liable for any damage resulting from handling or from contact with the above product.