Procell

Procell Cytogenetic Reagents

Cytoclear
Product Code: GGS-JL004

Cytoclear is a newly developed product (consisting of a mixture of protein detergents) which can quickly dissolve and remove persistent cytoplasm (mainly caused by inadequate hypotonic treatment) during the methanol:acetic acid fixation stage of the chromosome harvesting procedure. In particular, it dissolves the lipid and glycoprotein structure of which cytoplasm is mainly composed. Clumped metaphases that would normally be impossible to analyse can now be restored. Cytoclear is a useful tool in FISH hybridizations as it enables the probe to reach the target by removing barrier cytoplasm.

Hypotonic Comparisons

Pack size – 1ml vial

PRODUCT DESCRIPTION

Cytoclear is a newly developed product (consisting of a mixture of protein detergents) which can quickly dissolve and remove persistent cytoplasm (mainly caused by inadequate hypotonic treatment) during the methanol:acetic acid fixation stage of the chromosome harvesting procedure. In particular, it dissolves the lipid and glycoprotein structure of which cytoplasm is mainly composed. Clumped metaphases, which would normally be impossible to analyse, can now be restored

STORAGE
Store the product in a refrigerator for no more than 12 months.
Never heat or freeze.
For laboratory research use only.

PROCEDURE
There are two protocols for Cytoclear. In order to integrate Cytoclear into your laboratory as simply as possible, we recommend that you try Procedure 1 first.

PROCEDURE 1

  1. At the first fix step in the harvest, add 5ul of Cytoclear per ml of methanol:acetic acid (normally 3:1 ratio or whatever is the laboratory standard ratio), as normally used in your harvest procedure.
  2. Stand for 5 minutes
  3. Centrifuge for 5 minutes at 1000rpm
  4. Continue the harvest as normal.

PROCEDURE 2

For cultures that have yielded poor quality metaphases.

  1. Identify problem cultures by checking freshly made slides under phase contrast
  2. Take the problem culture, add 5ul of Cytoclear per ml of methanol: acetic acid (normally 3:1 ratio or whatever is the laboratory standard ratio), as normally used in your harvest procedure to the resuspended pellet.
  3. Stand at RT for 10 minutes.
  4. Spin down, then refix.
  5. Make slides

QUALITY CONTROL
All batches are tested for its ability to break down glycoprotein cytoplasm and are assessed using phase contrast microscopy.

MATERIAL SAFETY DATA SHEET
Date of issue 5th May 2005

IDENTIFICATION OF THE SUBSTANCE AND OF THE COMPANY

Catalogue Number: GGS-JL-004 - Cytoclear

Genial Genetic Solutions Ltd
Champions Business Park, Arrowe Brook Road, Upton, Wirral, CH49 0AB United Kingdom
Tel: +44 (0)1244 404 970
Fax: +44 (0)1244 404 971
Email: info@genialgenetics.com
Web: www.genialgenetics.com

COMPOSITION AND COMPONENT DATA
Proprietary product containing protein solubilising surfactants and enzymes dissolved in phosphate buffered saline.

HAZARDS IDENTIFICATION
Extremely harmful with eye contact. Protective glasses must be worn when using Cytoclear at all times.

FIRST AID MEASURES
In case of eye contact flush eyes with copious amounts of water.
In case of skin contact immediately wash skin with soap and water.
If swallowed, wash out mouth with water and drink lots of fluids

FIRE FIGHTING MEASURES
Not flammable.

ACCIDENTAL RELEASE MEASURES
Wear a lab coat and rubber gloves, wipe with paper tissues and place in plastic bag for proper disposal.

HANDLING AND STORAGE
Keep container tightly closed when not in use.
Store in refrigerator away from any light
Never freeze.
Wash hands after handling.

EXPOSURE CONTROL AND PERSONAL PROTECTION
Only work in a Class 2 Biological Safety Cabinet.
Always wear a lab coat and rubber gloves

PHYSICAL AND CHEMICAL PROPERTIES
Appearance: clear liquid to slightly yellow
Boiling Point: As for water
Vapour Pressure (pascals): As for water
Specific Gravity: 1.2
Flashpoint: N/A
Flammability Limits (%): N/A

TOXICOLOGICAL INFORMATION
To the best of our knowledge, the chemical, physical and toxicological properties have not been thoroughly investigated. Not a known carcinogen.

ECOLOGICAL INFORMATION
Data is not yet available.

DISPOSAL CONSIDERATIONS
Use approved government contractor.
Observe all International, National, State and Local environmental laws.

TRANSPORT INFORMATION
Not applicable

REGULATORY INFORMATION
Not applicable

OTHER INFORMATION
The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. Genial Genetic Solutions Ltd shall not be held liable for any damage resulting from handling or from contact with the above product.