Procell Cytogenetic Reagents

Chromosome Resolution Additive
Product Code: GGS-JL003b (5ml)

A novel formulation which prevents chemical contraction and encourages chromosome elongation by loosening the bonding within the supercoiled intrachromosomal structure. Antagonistic to the supercontracting effects of colchicine exposure, CRA acts as a molecular wedge, causing the chromosome structure to elongate. CRA is specially developed for tissues resistant to existing prophase techniques, such as bone marrow and tumour samples. This product does not contain Ethidium Bromide or Thymidine. CRA does not induce chromosome breakage.

Pack size – 5 ml vial

PRODUCT DESCRIPTION

CRA (Chromosome Resolution Additive) is a newly discovered commercial formulation that consistently prevents chromosome contraction and encourages chromosome elongation by loosening the chemical bonding within the supercoiled structure of the chromosome. The result is prophase quality chromosomes. It was developed especially for tissues resistant to existing prophase techniques, such as bone marrows and solid tumors. CRA contains no ethidium bromide or thymidine.

STORAGE
Not for drug or other use
CRA is prepared in phosphate buffered saline sterile, filtered, and supplied in a stock solution consisting of a 1ml vial with a shelf-life of 12 months.
The stock solution should be stored in an air-tight container at 2-6 0C.
Never freeze this product.
For Laboratory research use only.

To make a working solution:
Dissolve 0.1ml of CRA stock in 9.9ml of injection quality water.
Only use 0.1ml of working solution per 10ml culture. Working solution must be used and never stored.
NOTE: Scientists can make up smaller working volumes of CRA but should always maintain the above ratio ie. 1 part CRA in 99 parts injection quality water

PROCEDURE

1. Add 0.1 ml of CRA (working solution) to each culture containing 10ml of culture medium (see note above).
2. Incubate at 37 °C for 90 mins (see note below).
3. Harvest as usual.

NOTES

• Lengthy exposure to Chromosome Resolution Additive can lead to a reduced mitotic index. We recommend that laboratories try the following timings:
  o Start with Chromosome Resolution Additive incubation for 90 mins prior to harvest.
  o This can be decreased down to 30 mins if desired.
• Chromosome Resolution Additive can be added pre or post colchicine.
• If laboratories want to leave Chromosome Resolution Additive in for much longer time periods, they should dilute, between a quarter to an eighth, the concentration of Chromosome Resolution Additive.

QUALITY CONTROL
Batch testing is done on cancer cell lines scoring chromosome length against a suitable control.

MATERIAL SAFETY DATA SHEET
Date of issue 5th May 2005

IDENTIFICATION OF THE SUBSTANCE AND OF THE COMPANY

Catalogue Number: GGS-JL-003/b - Chromosome Resolution Additive
For laboratory and research purposes only.

Genial Genetic Solutions Ltd
Champions Business Park, Arrowe Brook Road, Upton, Wirral, CH49 0AB United Kingdom
Tel: +44 (0)1244 404 970
Fax: +44 (0)1244 404 971
Email: info@genialgenetics.com
Web: www.genialgenetics.com

COMPOSITION AND COMPONENT DATA
An aqueous solution containing inorganic salts, urea, cell culture medium, and commercially sensitive components

HAZARDS IDENTIFICATION
Irritant

FIRST AID MEASURES
In case of eye contact, immediately flush eyes with copious amounts of water for at least 15 minutes.
In case of skin contact, immediately wash skin with soap and copious amount of water.
If swallowed, wash out mouth with water and drink lots of fluids.
Call a physician.

FIRE FIGHTING MEASURES
Not known to be flammable, but will emit toxic fumes under extreme heat.

ACCIDENTAL RELEASE MEASURES
Wear a lab coat and rubber gloves, wipe with paper tissues and place in plastic bag for proper disposal. Wash area where spill occurs three times with water.

HANDLING AND STORAGE
Keep container tightly closed when not in use. Store product at 4C
Correctly stored, the product is stable for 12 months.

EXPOSURE CONTROL AND PERSONAL PROTECTION
Only work in a Class 2 biological safety cabinet.
Always wear a lab coat and rubber gloves. Wash hands after handling.

PHYSICAL AND CHEMICAL PROPERTIES
Appearance: Red liquid
Boiling Point: As for water
Vapour Pressure (pascals): As for water
Specific Gravity: 1.2
Flashpoint: N/A
Flammability Limits (%): N/A
Incompatible with strong oxidising agents
Incompatible with acids
Incompatible with freezing

STABILITY AND REACTIVITY
Not applicable

TOXICOLOGICAL INFORMATION
Harmful if investigated.
Not a known swallowed, inhaled or absorbed through the skin.
May cause eye irritation. May cause skin irritation
To the best of our knowledge, the chemical, physical and toxicological properties have not been thoroughly investigated. Not a known carcinogen.

ECOLOGICAL INFORMATION
Data not yet available

DISPOSAL CONSIDERATIONS
Use approved government contractor.
Mix with combustible solvent and burn in a chemical incinerator equipped with afterburn and scrubber.
Observe all International, National, State and Local environmental laws.

TRANSPORT INFORMATION
Not applicable

REGULATORY INFORMATION
EUROPEAN INFORMATION
R 20/21/22 (Harmful by inhalation, skin contact and if taken by mouth)
S 36 (Wear suitable protective clothing)

OTHER INFORMATION
The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. Genial Genetic Solutions Ltd shall not be held liable for any damage resulting from handling or from contact with the above product.