Procell

Procell Cytogenetic Reagents

Buffered Hypotonic Solution
Product Code: GGS-JL006a (250ml)

Buffered Hypotonic Solution is a hypotonic solution developed for bone marrow specimens. It has been found to achieve good metaphase swelling (spreading) from a wide range of sample types and significantly increases the number of useful metaphases in leukaemic bone marrow cultures when compared to the standard 0.075M KCL hypotonic. It contains 0.4% KCL with HEPES.

Hypotonic Comparisons

Pack size – to make 250 mls

PRODUCT DESCRIPTION

Buffered Hypotonic Solution is a hypotonic solution developed for bone marrow specimens. It has been found to achieve good metaphase swelling (spreading) from a wide range of sample types and significantly increases the number of useful metaphases in leukaemic bone marrow cultures when compared to the standard 0.075M KCL hypotonic. It contains 0.4% KCL with HEPES.

STORAGE
Store the working solution at 4°C for no more than 2 weeks.
Powdered product should be stored for no longer than 12 months at room temperature.
Never heat or freeze.
For laboratory research use only.

PROCEDURE

  1. Add the powdered contents of the supplied bottle of Buffered Hypotonic Solution product to 250mls of distilled water.
  2. Adjust to pH 7.4 with 5N NaOH and store at 4°C in a clean glass bottle, until ready to use, noting down the date on the bottle.
  3. During harvest replace your existing hypotonic solution with Buffered Hypotonic Solution
  4. Replace your existing hypotonic and incubate cells at 37°C for a maximum of 30 minutes.

QUALITY CONTROL
All batches are tested on established lymphoid/myeloid cell-lines with spreading assessed using phase contrast microscopy.

MATERIAL SAFETY DATA SHEET
Date of issue 5th May 2005

IDENTIFICATION OF THE SUBSTANCE AND OF THE COMPANY

Catalogue Number: GGS-JL-006/a - Buffered Hypotonic Solution (Powder for 250mls)

Genial Genetic Solutions Ltd
Champions Business Park, Arrowe Brook Road, Upton, Wirral, CH49 0AB United Kingdom
Tel: +44 (0)1244 404 970
Fax: +44 (0)1244 404 971
Email: info@genialgenetics.com
Web: www.genialgenetics.com

COMPOSITION AND COMPONENT DATA
Proprietary product containing potassium chloride 0.075 Molar pH 7.4 with Hepes

HAZARDS IDENTIFICATION
Not harmful by inhalation, with skin contact or if swallowed.

FIRST AID MEASURES
In case of eye contact flush eyes with copious amounts of water.
In case of skin contact immediately wash skin with soap and water.
If swallowed, wash out mouth with water and drink lots of fluids.

FIRE FIGHTING MEASURES
Not applicable. Non flammable

ACCIDENTAL RELEASE MEASURES
Wear a lab coat and rubber gloves, wipe with paper tissues and place in plastic bag for proper disposal.

HANDLING AND STORAGE
Keep container tightly closed when not in use.
Store prepared working solution at 4°C

EXPOSURE CONTROL AND PERSONAL PROTECTION
Only work in a Class 2 Biological Safety Cabinet.
Always wear a lab coat and rubber gloves.
Wash hands after handling.

PHYSICAL AND CHEMICAL PROPERTIES
Appearance: soft white powder
Boiling Point: As for water (for prepared working solution)
Vapour Pressure (pascals): As for water (for prepared working solution
Specific Gravity: 1.4 (for prepared working solution)
Flashpoint: N/A
Flammability Limits (%): N/A
Incompatible with strong oxidising agents
Incompatible with acids
Incompatible with freezing

TOXICOLOGICAL INFORMATION
To the best of our knowledge, the chemical, physical and toxicological properties have not been thoroughly investigated.
Not a known carcinogen.

REACTIVITY DATA
Not applicable

ECOLOGICAL INFORMATION
Data not yet available

DISPOSAL
Use approved government contractor.
Observe all International, National, State and Local environmental laws.

OTHER INFORMATION
The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. Genial Genetic Solutions Ltd shall not be held liable for any damage resulting from handling or from contact with the above product.