Procell Cytogenetic ReagentsBone Marrow Growth Supplement
Product Code: GGS-JL002
A new media additive that has a marked stimulatory effect on cell growth and morphology in Bone Marrow samples by exposing the cells in culture to a complex mixture of known growth promoters. Also effective with solid tumour, lymphoma and products of conception samples. Benefits include a significant improvement in cell growth (even in very poor samples), improved morphology and therefore improved banding and decreased turnaround times.
Pack size – 20 ml
Bone Marrow Growth Supplement is a media additive that greatly improves cell growth and morphology in bone marrow cultures by exposing these cells to growth factors that are known growth promoters. Also effective with difficult-to-grow solid tumours, lymphomas and products of conception
Bone Marrow Growth Supplement
has a marked stimulatory effect on bone marrow cells. It is
a complex mixture of growth factors dissolved in DMEM
Advantages that will be seen in bone marrow cultures are the following:
- a significant improvement in cell growth, even in very poor samples
- improved morphology and GTG banding
- a decrease in turn-around times
Store refrigerated until used. Do not freeze.
Store away from light
For laboratory research use only
Add 1 part of Bone Marrow Growth Supplement to 10 parts of normal foetal calf supplemented culture medium (Hams F10, RPMI, DMEM). Do not use in greater concentrations, as a medium nor as a FCS replacement. Where possible two cultures should be set up per specimen, one supplemented with Bone Marrow Growth Supplement and one without.
All batches are tested on established cell lines, with mitotic index and morphology assessed using phase contrast microscopy. The chromosome morphology and band length are assessed by scoring for the presence of chromosome bands routinely observed in greater than 450 band cytogenetic preparations. The test lot must perform statistically better than the control.
MATERIAL SAFETY DATA SHEET
Date of issue 5th May 2005
IDENTIFICATION OF THE SUBSTANCE AND OF THE COMPANY
Catalogue Number: GGS-JL-002 Bone Marrow Growth Supplement
For laboratory and research purposes only
Genial Genetic Solutions Ltd
Champions Business Park, Arrowe Brook Road, Upton, Wirral, CH49 0AB United Kingdom
Tel: +44 (0)1244 404 970
Fax: +44 (0)1244 404 971
COMPOSITION AND COMPONENT DATA
Proprietary product (Patent pending): containing a mixture of growth factors dissolved in MEM media (20% FCS).
Growth factors contained include:
Interleukine-3, Interleukine-6, Colony Stimulating Factor
FIRST AID MEASURES
In case of eye contact flush eyes with copious amounts of water.
In case of skin contact immediately wash skin with soap and water.
If swallowed, wash out mouth with water and drink lots of fluids.
FIRE FIGHTING MEASURES
ACCIDENTAL RELEASE MEASURES
Wear a lab coat and rubber gloves, wipe with paper tissues and place in plastic bag for proper disposal.
HANDLING AND STORAGE
Keep container tightly closed when not in use.
Store product at 4°C
Correctly stored, the product is stable for 12 months.
EXPOSURE CONTROL AND PERSONAL
Only work in a Class 2 biological safety cabinet.
Always wear a lab coat and rubber gloves.
Wash hands after handling.
PHYSICAL AND CHEMICAL PROPERTIES
Appearance: clear pink-red liquid
Boiling Point: As for water
Vapour Pressure (pascals): As for water
Specific Gravity: 1
Flammability Limits (%): N/A
STABILITY AND REACTIVITY
To the best of our knowledge, the chemical, physical and toxicological properties have not been thoroughly investigated.
Not a known carcinogen.
Use government approved contractor.
Observe all International, National, State and Local environmental laws.
The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. Genial Genetic Solutions Ltd shall not be held liable for any damage resulting from handling or from contact with the above product.