Procell Cytogenetic Reagents, Lymphocyte Growth Supplement Protocol

LYMPHOCYTE GROWTH SUPPLEMENT PROTOCOL

CAT GGS-JL-002/L

Pack size – 10 ml

PRODUCT DESCRIPTION

Lymphocyte Growth Supplement is a media additive that greatly improves cell growth and morphology in blood cultures by exposing these cells to growth factors that are known growth promoters.

Lymphocyte Growth Supplement has a marked stimulatory effect on bloods. It is a complex mixture of growth factors dissolved in DMEM containing FCS.

Advantages that will be seen in blood cultures are the following:

STORAGE
Store refrigerated until used. Do not freeze.
Store away from light
For laboratory research use only

PROCEDURE
Add 1 part of Lymphocyte Growth Supplement to 100 parts of normal foetal calf supplemented culture medium (Hams F10, RPMI, DMEM). Do not use in greater concentrations, as a medium nor as a FCS replacement. When first using Lymphocyte Growth Supplement, if possible, two cultures should be set up per specimen, one supplemented with Lymphocyte Growth Supplement and one without.

QUALITY CONTROL
All batches are tested on established cell lines, with mitotic index and morphology assessed using phase contrast microscopy. The chromosome morphology and band length are assessed by scoring for the presence of chromosome bands routinely observed in greater than 450 band cytogenetic preparations. The test lot must perform statistically better than the control.

MATERIAL SAFETY DATA SHEET
Date of issue 5th May 2005

IDENTIFICATION OF THE SUBSTANCE AND OF THE COMPANY

Catalogue Number: GGS-JL-002/L - Lymphocyte Growth Supplement
For laboratory and research purposes only, cell growth promoter.

Genial Genetic Solutions Limited
The Heath Business and Technical Park, Runcorn, Cheshire WA7 4QX United Kingdom
Tel: +44 (0)870 757 9300
Fax: +44 (0)870 757 9301
Email: info@genialgenetics.com
Web: www.genialgenetics.com

COMPOSITION AND COMPONENT DATA
Proprietary product containing (Patent pending):
a mixture of growth factors dissolved in MEM media (20% FCS).
Growth factors contained include:
Interleukine-3, Interleukine-6, Colony Stimulating Factor
GM-CSF

HAZARDS IDENTIFICATION
Non-hazardous

FIRST AID MEASURES
In case of eye contact flush eyes with copious amounts of water.
In case of skin contact immediately wash skin with soap and water.
If swallowed, wash out mouth with water and drink lots of fluids.

FIRE FIGHTING MEASURES
Not flammable.

ACCIDENTAL RELEASE MEASURES
Wear a lab coat and rubber gloves, wipe with paper tissues and place in plastic bag for proper disposal.

HANDLING AND STORAGE
Keep container tightly closed when not in use.
Store product at 4°C
Correctly stored, the product is stable for 12 months.

EXPOSURE CONTROL AND PERSONAL PROTECTION
Only work in a Class 2 Biological Safety Cabinet.
Always wear a lab coat and rubber gloves.
Wash hands after handling .

PHYSICAL AND CHEMICAL PROPERTIES
Appearance: clear pink-red liquid
Boiling Point: As for water
Vapour Pressure (pascals): As for water
Specific Gravity: 1
Flashpoint: N/A
Flammability Limits (%):

STABILITY AND REACTIVITY

Stability:	Stable at 4°C for 12 months
Conditions to avoid:	Not available
Incompatibility:	Not available
Hazardous Decomposition:	Nil
By-products:	Not applicable
Hazardous Polymerisation:	Not available

TOXICOLOGICAL INFORMATION
To the best of our knowledge, the chemical, physical and toxicological properties have not been thoroughly investigated.
Not a known carcinogen.

ECOLOGICAL INFORMATION
Low concentration biological components, biodegradable

DISPOSAL CONSIDERATIONS
Use government approved contractor.
Observe all International, National, State and Local environmental laws .

TRANSPORT INFORMATION
Not applicable

REGULATORY INFORMATION

OTHER INFORMATION
The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. Genial Genetic Solutions Ltd shall not be held liable for any damage resulting from handling or from contact with the above product..