Procell Cytogenetic Reagents, Cytoclear Cytoplasm Remover

Cytoclear is a newly developed product (consisting of a mixture of protein detergents) which can quickly dissolve and remove persistent cytoplasm (mainly caused by inadequate hypotonic treatment) during the methanol:acetic acid fixation stage of the chromosome harvesting procedure. In particular, it dissolves the lipid and glycoprotein structure of which cytoplasm is mainly composed. Clumped metaphases, which would normally be impossible to analyse, can now be restored

STORAGE
Store the product in a refrigerator for no more than 12 months.
Never heat or freeze.
For laboratory research use only.

PROCEDURE
There are two protocols for Cytoclear. In order to integrate Cytoclear into your laboratory as simply as possible, we recommend that you try Procedure 1 first.

For cultures that have yielded poor quality metaphases.

QUALITY CONTROL
All batches are tested for its ability to break down glycoprotein cytoplasm and are assessed using phase contrast microscopy.

MATERIAL SAFETY DATA SHEET
Date of issue 5th May 2005

IDENTIFICATION OF THE SUBSTANCE AND OF THE COMPANY

Catalogue Number: GGS-JL-004 - Cytoclear

Genial Genetic Solutions Limited
The Heath Business and Technical Park, Runcorn, Cheshire WA7 4QX United Kingdom
Tel: +44 (0)870 757 9300
Fax: +44 (0)870 757 9301
Email: info@genialgenetics.com
Web: www.genialgenetics.com

COMPOSITION AND COMPONENT DATA
Proprietary product containing protein solubilising surfactants and enzymes dissolved in phosphate buffered saline.

HAZARDS IDENTIFICATION
Extremely harmful with eye contact. Protective glasses must be worn when using Cytoclear at all times.

FIRST AID MEASURES
In case of eye contact flush eyes with copious amounts of water.
In case of skin contact immediately wash skin with soap and water.
If swallowed, wash out mouth with water and drink lots of fluids

FIRE FIGHTING MEASURES
Not flammable.

ACCIDENTAL RELEASE MEASURES
Wear a lab coat and rubber gloves, wipe with paper tissues and place in plastic bag for proper disposal.

HANDLING AND STORAGE
Keep container tightly closed when not in use.
Store in refrigerator away from any light
Never freeze.
Wash hands after handling.

EXPOSURE CONTROL AND PERSONAL PROTECTION
Only work in a Class 2 Biological Safety Cabinet.
Always wear a lab coat and rubber gloves

PHYSICAL AND CHEMICAL PROPERTIES
Appearance: clear liquid to slightly yellow
Boiling Point: As for water
Vapour Pressure (pascals): As for water
Specific Gravity: 1.2
Flashpoint: N/A
Flammability Limits (%): N/A

TOXICOLOGICAL INFORMATION
To the best of our knowledge, the chemical, physical and toxicological properties have not been thoroughly investigated. Not a known carcinogen.

ECOLOGICAL INFORMATION
Data is not yet available.

DISPOSAL CONSIDERATIONS
Use approved government contractor.
Observe all International, National, State and Local environmental laws.

TRANSPORT INFORMATION
Not applicable

REGULATORY INFORMATION
Not applicable

OTHER INFORMATION
The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. Genial Genetic Solutions Ltd shall not be held liable for any damage resulting from handling or from contact with the above product.